Pharmacy

Colorimetric Determination of Tetracycline Hydrochloride with 4 - Nitrobenzenediazonium Hydrogen Sulphate

Tetracycline hydrochloride was coupled with 4 - nitrobenzenediazonium hydrgen sulphate in aqueous medium. Freshly prepared cold saturated solution of sodium acetate facilitated the coupling reaction at room temperature thus obviating the high temperature required for coupling in the method of Kakemi, Arita, Sezaki and Nadal(1) . The azo product in 0.1M sodium hydroxide solution had a wavelength of maximum absorption of 425nm and Emax of 27,800 L mol-1cm-1.

Department

Colorimetric Determination of Zidovudine in Bulk and Tablet Dosage Form Using Potassium Dichromate

A colorimetric method involving the oxidation of zidovudine (ZDV) using potassium dichromate in sulphuric acid has been developed for the determination of ZDV in bulk and tablet dosage forms. The wavelength of maximum absorption (λmax) of the oxidation product was determined and concentrations of sulphuric acid, potassium dichromate and amount/duration of heat were optimized. Beers plot was constructed at the λmaxand used in the recovery studies for pure drug sample. Two methods (acid and methanol extraction) were used to extract ZDV from tablet samples.

Department

Extraction and Fractionation of Whole Seed Plantain ( Plantago rugelii Decne) for in vitro antioxidant, antibacterial and Phytochemical Screening

This study was designed to explore new antioxidant and antimicrobial agents from the methanol whole plant crude extract and fractions of Plantago rugelii. The methanol extract and its fractions were prepared and screened for its phytochemical composition, in-vitro antioxidant potential and challenged with common pathogenic microorganisms for its antimicrobial activities using standard procedures.

Department

Phenolic Compounds from Endophytic Pseudofusicoccum Sp Isolated from Annona muricata

Endophytic fungal populations of Nigerian medicinal plants have been shown to possess enormous potentials as sources of biologically active compounds of pharmaceutical and industrial importance. Our study was carried out to investigate the secondary metabolites of an endophytic fungus Pseudofusicoccum sp. isolated from the leaves of Annona muricata growing in Ifite Dunu, Anambra State, South-East Nigeria.

Department

Secondary Metabolites fromConvularia Sp. An Endophytic Fungus Isolated from the Leaves of Picralima nitida Durand and Hook (Apocynaceae)

Nature has remained a major source of pharmacologically active compounds used for the treatment of new and existing diseases, or as lead molecules for the development of synthetically derived analogues. This research was carried out to determine the secondary metabolites from the extract of Curvularia sp, an endophytic fungus associated with the leaves of Picralima nitida. The endophytic fungus was isolated and purified from the leaves of the plant material, using the conventional methods.

Department

Endophytic Fungi as Alternative and Reliable Sources of Anti-Cancer Agents. Natural Products and Cancer Drug Discovery

In comparison with other natural sources like plants, highly diverse microorganisms are narrowly explored, especially with respect to their limitless potentials as repositories of exceptionally bioactive natural products. Of these organisms, fungi inhabiting tissues of plant in a noninvasive relationship (endophytic fungi) have proven undeniably useful and unmatchable as sources of potent bioactive molecules against several diseases such as cancer and related ailments.

Department

Extractive Ion Pair Spectrophotometric Assay of Quinine

Quality control and assurance of antimalarials is vital in malaria treatment success. This research developed an extractive spectrophotometric method for routine assay of quinine, for enhanced quality control and assurance. The developed method is based on the formation of coloured ion-pair complex between quinine and bromocresol green in phthalate buffer, pH 5. The ion-pair was extracted into chloroform and re-extracted into 0.1M sodium hydroxide. The 0.1M sodium hydroxide extract was determined at 620nm.

Department

Cytotoxicity and antimicrobial activities of secondary metabolites produced by endophytic fungi isolated from Gongroneme latifolia and Loranthus micrantus

This study was carried out to evaluate the cytotoxicity and antimicrobial properties of secondary metabolites produced by endophytic fungi isolated from Gongronema latifolia and Loranthus micranthus. Isolation of endophytic fungi from plant leaves was carried out using a previously described method. Solid state fermentation was carried out in rice medium for 30 days at 25 – 27 °C and the secondary metabolites were extracted using ethyl acetate. Cytotoxic effects of the extracts were tested on mouse lymphoma cell line (L5178Y) using MTT assay.

Department